Enhancing Drought Tolerance in Canola, Brassica napus

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With the ever-increasing occurrences and intensities of drought relating to global climate change (Cook et al. 2014, Singh et al. 2015) crops are negatively affected, limiting the ability for high yields. One such crop is canola, Brassica napus. Canola is a key crop plant in some of the world's top industries, including food and renewable energy. Unfortunately, canola is not a drought tolerant plant, which can impact its supply of food, feed, and research into renewable energy (Zhu et al., 2016, Wang et al. 2016, Tarinejad et al., 2013). Because of these factors it is vital to transform canola into a drought tolerant crop plant. By using agrobacterium mediated hypocotyl explants transformation to place the KfNAC83 vector into the canola genome, we have the potential to produce the highest yield of drought tolerant canola. Previous research has shown increased drought tolerance and biomass mass growth when overexpressed in Arabidopsis thaliana (Amin et al. 2019). This is done by the process of excising hypocotyl explants and preculturing them. Then the explants are inoculated with agrobacterium suspension and returned to a callus induction medium for cocultivation. Once lines of transgenic canola carrying the KfNAC83 TF are produced, we will assay the transgenic canola for drought tolerance. Obtaining the genetically modified, drought tolerant plant, might be advantageous in the food and renewable energy industries in a projected drier environment.

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Bernie Wone

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