Date of Award

Summer 8-28-2020

Document Type

Honors Thesis

Department/Major

Biology

First Advisor

Victor Huber

Second Advisor

Karen Koster

Third Advisor

Beate Wonne

Keywords

Influenza, Ferrets, Kinetics, TCID50

Subject Categories

Diseases | Immunology and Infectious Disease

Abstract

Influenza is a persistent threat to individual and global health. Seasonal influenza kills nearly 500,000 people globally each year. Influenza viruses have circulated within the human population, causing a significant disease burden. In order to create the most effective antiviral therapies, knowledge concerning all lineages of influenza must be studied accurately and thoroughly. The influenza virus itself is not always responsible for these mortalities, rather, the secondary bacterial infections associated with death. In order to protect the population and examine the secondary infections that arise, the kinetics of influenza must be studied entirely. To understand the kinetics, researchers are turning to the use of laboratory experimentation using ferrets as an effective model. This is done through the use of common laboratory procedures such as TCID50’s and hemagglutination assays. Through the use of these procedures, the kinetics of influenza A and B can be confirmed. Within this study, it was confirmed that influenza A infects both the upper and lower respiratory system, while influenza B infects the upper respiratory tract but fails to reach the lower lungs. Understanding the kinetics of influenza is the first step toward creating effective therapies to reduce the mortality and illness associated with the influenza virus infection.

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