Toxic Effect of Crotalus adamanteus Acidic Phospholipase A2 on MCF-7 Cell Line

Author

Daniel J. Petra, University of South DakotaFollow

Date of Award

Spring 4-7-2021

Document Type

Honors Thesis

Department/Major

Basic Biomedical Science

First Advisor

Victor Huber

Second Advisor

Khosrow Rezvani

Third Advisor

David Hawkinson

Keywords

Crotalus adamanteus, phospholipase a2, mcf-7

Subject Categories

Amino Acids, Peptides, and Proteins | Cancer Biology | Enzymes and Coenzymes | Laboratory and Basic Science Research

Abstract

We are investigating the effect of Crotalus adamanteus acidic phospholipase A₂ on MCF-7 cells using the MTS assay. Understanding these interactions and isolated effects is critical to developing new ways to treat envenomation. By understanding the effects of individual toxins within a whole venom, we are set to better understand the effects of the whole venom and investigate synergistic actions between venom toxins. In this paper, we are quantifying the amount of MCF-7 cell death caused by Crotalus adamanteus phospholipase A₂ on MCF-7 using the MTS assay. Analysis of the amount of cells death caused by the phospholipase suggests that it may be responsible for as much as 87% of the whole venom’s cytotoxic activity.

Recommended Citation

Petra, Daniel J., "Toxic Effect of Crotalus adamanteus Acidic Phospholipase A2 on MCF-7 Cell Line" (2021). Honors Thesis. 129.
https://red.library.usd.edu/honors-thesis/129