Date of Award
Basic Biomedical Science
Crotalus adamanteus, phospholipase a2, mcf-7
Amino Acids, Peptides, and Proteins | Cancer Biology | Enzymes and Coenzymes | Laboratory and Basic Science Research
We are investigating the effect of Crotalus adamanteus acidic phospholipase A2 on MCF-7 cells using the MTS assay. Understanding these interactions and isolated effects is critical to developing new ways to treat envenomation. By understanding the effects of individual toxins within a whole venom, we are set to better understand the effects of the whole venom and investigate synergistic actions between venom toxins. In this paper, we are quantifying the amount of MCF-7 cell death caused by Crotalus adamanteus phospholipase A2 on MCF-7 using the MTS assay. Analysis of the amount of cells death caused by the phospholipase suggests that it may be responsible for as much as 87% of the whole venom’s cytotoxic activity.
Petra, Daniel J., "Toxic Effect of Crotalus adamanteus Acidic Phospholipase A2 on MCF-7 Cell Line" (2021). Honors Thesis. 129.