Date of Award

Spring 4-27-2026

Document Type

Honors Thesis

Department/Major

Biology

First Advisor

Victor Huber

Second Advisor

Daniel Bird

Third Advisor

Beate Wone

Keywords

macrophage, cytokine, phagocytosis, opsonophagocytosis, SRBC, S. mutans, uptake

Subject Categories

Medical Immunology | Oral Biology and Oral Pathology

Abstract

We are all exposed to numerous pathogens every day. One of the sites for pathogens to interact with our body is in the mouth. To better understand these interactions between bacteria and host immune cells, I developed an assay to study the oral bacteria Streptococcus mutans and inflammatory macrophages. Our hypothesis was that the cytokines IL-4 and IFN-y would alter macrophage phenotypes with IL-4 reducing inflammatory responses and IFN-y increasing inflammation.  To test this, we performed experiments in cell culture as a first step toward understanding the severity of the host response at the cellular and molecular levels. Our results indicate that macrophage phenotypes are indeed affected, particularly within 1-24 hours after inoculation. Additional experiments are necessary to further understand our results and draw more comprehensive conclusions.

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